CURRICULUM VITAE

Charles M Herr, BSc, PhD

General Information

PERSONAL  
Name: Charles Marvin Herr
Home Address: 1016 S. Main, Colfax, WA 99111
Work Address: Department of Biology, MS# Sci 258,

Eastern Washington University, Cheney, WA 99004
Phone Numbers: Home: 1-509-397-4968 Work: 1-509-359-2038
Emails: Home: charlieherrr@gmail.com
   
EDUCATION University of Wyoming

Laramie, Wyoming

 1976-79. BSc

Biology

  Washington State University

Pullman, WA

 1982-86, Ph.D.

Genetics and Cell Biology
Australian National University

Canberra, ACT, Australia

 1987-90. Visiting Fellow (post-doc equivalent)

Biochemistry Department

PROFESSIONAL WORK EXPERIENCE

PERIOD
ORGANIZATION
MAJOR ACTIVITY
1994-

currently

 Department of Biology, Eastern Washington University Started as Assistant Professor, tenure-track. Promoted to Associate Professor, tenured. I developed and teach the courses: cell biology, premed seminar and embryology; also, genetics, intro cell biology and others. I am a premed advisor. I direct graduate student research in integrative biology with a focus on cell biology and reproduction.
1994 Ethrog Biotechnology Ganot, Israel Consultant: When EB was a startup, I helped initiate research activities. EB was a successful biotech company. EB is now part of Invitrogen, a large international biotechnology company.
1992-

1993

 Department of Genetics and Cell Biology, Washington State University Adjunct Professor: Directed the graduate research of Lance Hilgers, who worked for me at AB Technology, USA, in the R & T Park at WSU. This was concurrent with the ABT position.
1987-

1993

 AB Technology, ANU, Canberra, Albury, NSW, and Pullman, Washington, USA Started as Research Leader, Embryology; promoted to Business Unit Manager, then to Chief Research and Development Scientist: Directed research, technology and product development; established new corporate facilities, and led the company's strategic management policy. Served on the board. The company created state-of-the-art biotechnologies for use on mammalian species. AB Technology is now part of Bioniche International, a large animal biotechnology company.
1987-

1993

 Riverina Artificial Breeders

Albury, New South Wales

 Consultant: RAB was bought byABT and became one of the world's largest breeding companies. I managed the integration of research advances. These included collection and transfer of embryos and semen from cattle, sheep, goats and deer. I integrated research with commercial activities. This was concurrent with the ABT position. RAB is now part of Genus, the largest artificial breeding company on the planet.
1987-

1990

 Department of Biochemistry, Australian National University Visiting Fellow: Research was on the application of isolated Y-chromosome repeats for pre-implantation sex determination; and, mammalian cloning technology. This position is equivalent to a post-doc. This position was concurrent with the AB technology position.
1985-

1987

 Scientific Automated Graphic Arts Owner: Created a computer graphics company. It serviced the University of Idaho and Washington State University. From the date you can see this would have been one of the first computer graphics companies in the world.
1982-

1986

 Department of Genetics and Cell Biology, Washington State University Graduate Student, Teaching Assistant and Research Assistant: Tandem duplication of DNA and mammalian embryo research. Gained experience in microbiology, cryobiology, cell culture and micromanipulation technology.
1981 Department of Animal Sciences, University of Idaho Technologist: Cattle nutrition studies on absorption of nutrients relative to the rumen. Activities ranged from weighing cows to studying microorganisms.
1977-

1981

 Department of Zoology, University of Wyoming Started as Technician I, promoted to Scientist I: cytogenetics, aging and mutagenesis research. Organism was Paramecium tetraaurelia. I was lab supervisor as well. There were about 10 lab workers.

 

TEACHING EXPERIENCE AND INTEREST
RESEARCH INTEREST AND ACTIVITIES AT EASTERN
INDUSTRY EXPERIENCE - RESEARCH AND MANAGEMENT
VARIOUS HONORS
SERVICE TO EASTERN’S STUDENTS, DEPARTMENT, UNIVERSITY AND THE SPOKANE COMMUNITY WHILE EMPLOYED AT EASTERN

 
  • NOTE: I put a point in front of all of the entries below that occurred from the year of my employment at Eastern Washington University and subsequently.

REFEREED PUBLICATIONS

Smith-Sonneborn, J., Herr, C. and Van Kirk, E. (1980) Paramecium tetraurelia prescreen for hazardous agents, Sci. Tech. Aerosp. Rep. 18, 2612a.

Smith-Sonneborn, J., Palizzi, R.A., Fisher, G.L. and Herr, C. (1981) Mutagenicity of fly ash particles in Paramecium, Science 211, 180.

Smith-Sonneborn, J., Fisher, G.L., Palizzi, R.A. and Herr, C. (1981) Mutagenicity of coal fly ash: A bioassay for mutagenic potential in a particle feeding ciliate, Environ. Mut. 3, 239.

Herr, C. and Wright, R.W., Jr. (1985) Cold storage (5°C) of mouse embryos in two different media, J. Anim. Sci. 61 (supp.1), 410a.

Herr, C. and Wright, R.W., Jr. (1986) Storage life of Whitten's embryo culture medium, Theriogenology 25, 158a.

Herr, C. and Wright, R.W., Jr. (1986) Long-term culture of hatched bovine embryos, Theriogenology 25, 159a.

Herr, C. (1986) "Factors influencing survival of preimplantation stage mouse embryos maintained in developmental arrest in vitro", Ph.D. thesis, Washington State University, Pullman WA (Published: University Microfilms International, Ann Arbor MI, 1987).

Herr, C. and Wright, R.W., Jr. (1988) Cold storage of mouse embryos of different stages of development, Theriogenology 29, 765.

Herr, C. and Wright, R.W., Jr. (1988) Cold-culture of different stage mouse embryos in bicarbonated and bicarbonate-free medium, Theriogenology 30, 159.

Bradley, M.P., Matthaei, K.I., Herr, C.M., Beaton, S. and Reed, K. (1990) The 50% fraction of bovine spermatozoa which selectively binds phytohaemagglutinin (PHA-L) does not correlate with sex genotype, Theriogenology 33, 199.

Herr, C., Holt, N., Petrzak, U., Old, K. and Reed, K. (1990) Increased number of pregnancies per collected embryo by bisection of blastocyst stage ovine embryos, Theriogenology 33, 244.

Herr, C.M., Matthaei, K.I., Petrzak, U. and Reed, K.C. (1990) A rapid Y-chromosome-detecting ovine embryo sexing assay, Theriogenology 33, 245.

Herr, C.M., Matthaei, K.I., Steel, T. and Reed, K.C. (1990) Rapid Y-chromosome-assay sexing of peripheral blood lymphocytes from Bovinae of known phenotypic sex, Theriogenology 33, 246.

Herr, C.M., Holt, N.A., Matthaei, K.I. and Reed, K.C. (1990) Sex of progeny from bovine embryos sexed with a rapid Y-chromosome-detection assay, Theriogenology 33, 247.

Matthaei, K.I., Herr, C.M., Bradley, M.P. and Reed, K.C. (1990) Analysis of the sex chromosome constitution of single bovine spermatozoa by polymerase chain reaction (PCR), Theriogenology 33, 285.

Herr, C.M., Matthaei, K.I. and Reed, K.C. (1990) Rapid, accurate sexing of livestock embryos, in Breeding Towards the Year 2000, Proceedings of 4th World Congress on Genetics Applied to Livestock Production (Edinburgh UK) 4, 186.

Herr, C.M. and Reed, K.C. (1991) Micromanipulation of bovine embryos for sex determination, Theriogenology 35, 45.

Hilgers, L.J. and Herr, C. (1993) DNA contamination of reagents used in embryo transfer and culture. Theriogenology, (November 1993 issue).
  • Hilgers, L.J. and Herr, C. (1994) Proteolytic digestion of genomic samples followed by chelation inactivation prior to PCR amplification. BioTechniques 16, 37.
  • Greeson, K. and Herr, C. (1998) An extremely sensitive silver staining technique useful for salvaging the results of preimplantation genetic diagnosis. Theriogenology 49, 272.
  • Kegerreis K.A. and Herr, C. (1998) Mouse H-Y antibody fails to detect the sex of human lymphocytes. Theriogenology 49, 158.
  • Oudes, A., Herr, C. Olsen, Y. and Fleming, J. (1998) Age-dependent accumulation of advanced glycation end-products in adult Drosophila melanogaster. Mechanisms of Aging and Development 100, 221.
  • Provo M.B. and Herr C. (1998) Washed paraffin oil becomes toxic to mouse embryos upon exposure to sunlight. Theriogenology 49, 214.
  • Oudes, A. and Herr, C. (1999) Resolving small advanced glycation end-products (AGE) using horizontal acrylamide gel electrophoresis and its usefulness for determining the efficacy of potential AGE inhibiting drugs. FASEB Meeting ’99 Abstract. Journal of Biochemistry and Molecular Biolology .Vol 13, No. 7, Abstract #963.
  • C. Valentin, R. West, C. Herr (2008) THE ENHANCEMENT OF THE SENSITIVITY OF A PCR-BASED AVIAN SEX DETERMINATION ASSAY. Journal of Reproduction, Fertility and Development 20, 214.
  •  R. West, C. Valentin, C. Herr (2008) STILL GLOWING AFTER ALL THESE YEARS: STORAGE LIFE OF A COMPLETE BOVINE SEXING PCR MIX. Journal of Reproduction, Fertility and Development 20, 215.
  • C. Herr, C. Valentin, K. Greeson (2008) SUITABILITY OF BOVINE SEXING PRIMERS FOR SEX DETERMINATION OF CAPRINE CELLS Journal of Reproduction, Fertility and Development 20, 212.
  • B. K. Hopkins, C. Herr (2008) CRYOPRESERVATION OF FROG (RANA PIPIENS) SPERM CELLS COLLECTED BY NON-LETHAL METHODS. Journal of Reproduction, Fertility and Development 20, 120.
  • M. Stucky, B. K. Hopkins, C. Herr (2008) CRYOPRESERVATION OF HONEY BEE SPERMATOZOA. Journal of Reproduction, Fertility and Development 20, 127.

ADDITIONAL PUBLICATIONS

Herr, C., Lascelles, A. and Reed, K.C. (1989) "Splitting of Livestock Embryos. An Instruction Manual" (Advanced Riverina Holdings Ltd., Sydney).

Herr, C., Steel, T., Lascelles, A. and Reed, K.C. (1989) "Splitting, Biopsy and Sexing of Cattle Embryos. An Instruction Manual" (Advanced Riverina Holdings Ltd., Sydney).

Herr, C. (1992) Embryo Sexing, in Advanced Hands-On Workshop in Embryo Micromanipulation and Genetic Evaluation, p 71-90. American Fertility Society, Postgraduate Course.

Herr, C. (1992) Embryo Bisection for Intentional Production of Twins, in Advanced Hands-On Workshop in Embryo Micromanipulation and Genetic Evaluation, p.138-149. American Fertility Society, Postgraduate Course.

Herr, C. (1993) Embryo Sectioning: A Method for Increasing the Number of Progeny From a Finite Number of Embryos, in American Society of Andrology - Laboratory Science Forum - Workshop on Micromanipulation.

Herr, C. and Reed, K.C. (1993) "Splitting, Biopsy and Rapid Sexing of Cattle Embryos. An Instruction Manual" Second Edition (A.B. Technology, Inc., Pullman, Wa)

PATENTS & TRADEMARKS

Reed, K.C., Lord, E.A., Matthaei, K.I., Mann, D.A., Beaton, S., Herr, C.M. and Matthews, M.E. (1995) Determination of genetic sex in ruminants using Y-chromosome-specific polynucleotides, United States Patent: 5459038

Herr, C.M. (1989) Temperature maintenance in rechargeable insulated vessels based on the principle of latent heat of melting/fusion, Patent Applic. No. PJ4779/89. Patent has been granted, I do not have the current identifying symbols.

Herr, C.M. (1989) Temperature maintenance of animal and human semen, Document No. AU-B-45841/89 Patent Acceptance No. 600645.

Vigro_ series Trade Mark, Aust. Applic. No. 516182. Trademark has been granted, I do not have the current identifying symbols.

ConTemPak_ series Trade Mark, Aust. Applic. No. 516183. Trademark has been granted, I do not have the current identifying symbols.

Serile_ series Trade Mark, Aust. Applic. No. 516184. Trademark has been granted, I do not have the current identifying symbols.

RBX-tend_ Trade Mark, Aust. Applic. No. 516473. Trademark has been granted, I do not have the current identifying symbols.

GRADUATE STUDENTS WHOSE RESEARCH I DIRECTED AT EASTERN WASHINGTON UNIVERSITY

2007
The Molecular Sex Determination of Avian Species
Ryan West

								

2006
Cryopreservation techniques for the spermatozoa of honey bees (Apis mellifera) and leopard frogs (Rana pipiens)	
Michael C. Stucky
		

2003
Ultraviolet light causes fewer mutations at -198°C than 23°C
Stephanie Lynn Bernards


2000
Improvements in the success of embryo cryopreservation may be achieved by the inclusion of sucrose in the freezing medium increasing embryo tolerance
Mary Beth Provo 

1999
New developments in advanced glycation end product detection and formation
Asa J. Oudes

									
1998
Mouse H-Y antibody fails to detect the sex of human lymphocytes
Kimberly A. Kegerreis
1997
Improvements in the use of PCR and gel electrophoresis for preimplantion 
genetic analysis
Kay A. Greeson (Sigafoos)


								 
CURRENT GRADUATE STUDENTS

Brandon Hopkins

Catlin Valentin

Amanda Mattheson

						

						

						
CONFERENCE PRESENTATIONS

					

					
Matthews, M.E., Matthaei, K.I., Herr, C. and Reed, K.C. (1987) Sex determination of pre-implantation livestock embryos, Proc. Aust. Soc. Reprod. Biol. (Sydney NSW) 19, 4.

					
Herr, C., Holt, N. and Reed, K.C. (1988) Effect of sucrose and calcium in the splitting medium on survival of quartered ovine morulae, Proc. Aust. Soc. Reprod. Biol. (Newcastle NSW) 20, 10a.

					
Herr, C., Matthaei, K.I. and Reed, K.C. (1989) Accuracy of a rapid Y-chromosome-detecting bovine embryo sexing assay, Int'l Embryo Transfer Soc. (San Diego CA).

					
Herr, C., Matthaei, K.I., Holt, N. and Reed, K.C. (1989) Field implementation of a rapid Y-chromosome-detecting bovine embryo sexing assay, Int'l Embryo Transfer Soc. (San Diego CA).

					
Herr, C., Matthaei, K.I. and Reed, K.C. (1989) Accuracy of a rapid Y-chromosome-detecting bovine embryo sexing assay, Proc. ANZ Soc. Cell Biol. (Melbourne VIC) 8, 50a.

					
Herr, C., Matthaei, K.I., Holt, N. and Reed, K.C. (1989) Field implementation of a rapid Y-chromosome-detecting bovine embryo sexing assay, Proc. ANZ Soc. Cell Biol. (Melbourne VIC) 8, 51a.

					
Bradley, M.P., Matthaei, K.I., Herr, C.M. and Reed, K.C. (1989) An evaluation of SemPrep_ in the enrichment of substandard semen samples for highly motile bovine spermatozoa, Proc. Conf. Animal Health and Production in the 21st Century (Sydney NSW) p. 22.

					
Herr, C.M., Matthaei, K.I. and Reed, K.C. (1989) Application of PCR to rapid sexing of bovine embryos, Proc. Aust. Biochem. Soc. (Southport QLD) 21, C25.

					
Lord, E.A., Matthaei, K.I., Herr, C. and Reed, K.C. (1990) Progeny testing embryos, Proc. Aust. Assoc. Animal Breeding Genet. (Palmerston North NZ) 8, .

					
Herr, C.M., Matthaei,K.I. and Reed, K.C. (1990) Embryo sexing in livestock by simultaneous PCR amplification of Y-chromosomal and autosomal DNA, Genetics Soc. Aust. (Adelaide SA).

					
					
					

						
							
								
INVITED LECTURES & INVITED PARTICIPATION

							
						

					
"Application of rapid DNA sequence detection technology to domestic species reproduction", in Animal Genetic Engineering W' shop, Aust. Biotech. Assoc. Conf. (Sydney NSW) 8, Feb. 1989.

					
"Application of sexing technology to the animal breeding industries", Int'l Embryo Transfer Soc. (Denver CO) Jan. 1990.

					
Panel discussion on ‘Patenting of animals’, Int'l Embryo Transfer Soc. (Denver CO) Jan. 1990.

					
Workshop on ‘Livestock embryo cloning’, Int'l Embryo Transfer Soc. (Denver CO) Jan. 1990.

					
"Cloning of Y-Chromosome Specific Sequences and Development of the First PCR-Based Sexing Assay".  University of Wyoming.  April, 1990.

					
Appearance on "BEYOND 2000" an Internationally broadcasted television program featuring futuristic new technologies. March 1990.

					
Symposium on 'The use of micromanipulation in commercial embryo transfer', Int'l Embryo Transfer Soc. (Bournemouth, ENGLAND) Jan. 1991.

					
Symposium on Practical Gamete and Embryo Micromanipulation. Sydney IVF. Title: "The microenvironment under the microscope." November 10, 1991.

					
Faculty for workshop 'Advanced hands-on workshop on embryo micromanipulation and genetic evaluation' American Fertility Society (University of Wisconsin-Madison) May 18-22, 1992.

					
Faculty for workshop 'Laboratory Science Forum Workshop on Micromanipulation' American Society of Andrology (Tampa, Florida) April 16, 1993.

					
					
    
						
  • Invited Seminar presentation, University of Montreal, Saint-Hyacinthe, Quebec, June, 1993
						
  • Invited Seminar presentation, University of Tel Aviv; Tel Aviv, Israel. May, 1994.
						
  • Invited Seminar presentation, University of Jerusalem; Red Sea Research Station, Ellot, Israel. July, 1994.
						
  • Invited Seminar presentation, SIRTI, Title: "Commercializing Biological Research". November, 1994.
						
  • Judge for "Scientific Olympiad", Washington StateWide. A science talent and knowledge competition for High School students; I judged the area of genetics. April, 1995.
						
  • Judge for "Scientific Olympiad", Washington StateWide, Eastern Washington. . A science talent and knowledge competition for High School students; I judged the area of genetics. March, 1996.
						
  • Judge for "Scientific Olympiad", Regional, Gonzaga University, Spokane WA. A science talent and knowledge competition for High School students; I judged the area of genetics. February, 1997.
						
  • Judge for "Scientific Olympiad", Washington StateWide. A science talent and knowledge competition for High School students; I judged the area of genetics. April 12, 1997.
						
  • Invited Seminar Presentation, Sigma Xi Eastern Washington University - Spokane Chapter. Title: "Advanced Technologies on the Range". April 23, 1997.
						
  • Speech to the Lions: Mammalian Cloning: What, How, and Where it might lead us., February 10, 1998.
						
  • Volunteer Service at Cheney Middle School: "How Dolly was made." Teaching for three days at the middle school. April, 1998.
						
  • Event Supervisor for "Scientific Olympiad", Regional, Eastern Washington University. . A science talent and knowledge competition for High School students; I judged the area of genetics. February, 1999.
						
  • Event Supervisor for "Scientific Olympiad", Washington StateWide. . A science talent and knowledge competition for High School students; I judged the area of genetics. April 12, 1999.
						
  • Event Supervisor for "Scientific Olympiad", Regional, Eastern Washington University. . A science talent and knowledge competition for High School students; I judged the area of genetics. February, 1999.
						
  • Invited Participation, Sinauer Associates and W.H. Freeman and Company, "Focus on the Future of General Biology Courses". North Hampton, Massachusetts, September 7-11, 1999.
						
  • Event Supervisor for "Scientific Olympiad", Regional, Spokane Community College, Spokane, Washington. A science talent and knowledge competition for High School students; I judged the area of Bioprocess. February, 2000.
						
  • Event Supervisor for "Scientific Olympiad", Washington StateWide. A science talent and knowledge competition for High School students throughout the state; I judged the area of Bioprocess. April 12, 2000.
						
  • Event Supervisor for "Scientific Olympiad", National Contest. A science talent and knowledge competition for High School students throughout the nation; I judged the area of Bioprocess. April 12, 2000.
						
  • Appeared on television, Channel 4, KREM, a regional nightly news program, to explain DNA fingerprinting as it related to the Spokane serial killer suspect. May, 2000.
						
  • Appeared on regional talk radio, KREM, to explain the human genome project to listeners. June 27, 2000.
						
  • Continue to serve as an event supervisor for "Scientific Olympiad" periodically.
						
  • Herr, C. (November 16, 2007) Seminar Presentation, Washington State University, Department of Entomology, Title: "Cryopreservation and Its Application to Bee Semen Longterm Storage".
					

					

						

						
TO ACCESS THE LIST OF REFEREES, CLICK THIS LINK
					

						
				
				
				
				
				
				
				
				
				
			

			

				All photos are by c. herr. Please site me if you use them.
				
			

			

				
				
			

			

				
				
			

			

				The first animals in the world to be sexed using the polymerase chain reaction on an embryonic cell were cattle. In fact this is a photo of the calves. We biopsied, sexed and "twinned" them in 1987.
					

					

						
				
				
			

			

				
				
			

			

				
				
			

			

				This is a photo of us field embryo sexing. Here, we had all the technology and equipment necessary to collect, find, biopsy, split, freeze and sex cattle embyos; all set up on the farmer's kitchen table. Much of the technolgy we created. We nicknamed the system the Swiss Army Lab. We could set it up in 20 minutes!
					

					

						
				
			

			

				
				
				
				
				
				
				
			

			

				This is Lance Hilgers making reaction mix, which we sold around the world. We were the first company in the world to have a license for the use of the polymerase chain reaction. We had the first commercial PCR kit for specific uses. The mixture that Lance is making sold for $20 per 10 ul!
				
			

			

				
				
			

			

				Technology or knowledge in general is useless unless disseminated. Here I am training scientists and veterinarians in Quebec how to split, biopsy and sex cattle embryos.
					

					

						
				
			

			

				
				
				
				
				
				
				
				
				
				
			

			

				I knew these little lambs and the other two just like them (clones) when they were just little cells.
					

					

						
				
			

			

				
				
				
				
				
				
			

			

				Students at Eastern have had the opportunity to learn how to grow mammalian embryos like these above. The clear broken ball is the "shell" mammalian embryos must hatch from after about 7 days of development.
					

					

						
				
			

			

				
				
				
				
				
				
				
				
			

			

				Research is not work at all! Its like getting paid to play. However, it hasn't been easy to find enough time. Above is a schematic of the sexing assay my colleagues and I developed. 
					

						
				
			

			

				
				
			

			

				
				Mouse model is cost effective for mammalian embryology research. Many of my students use this system.
				
			

			

				
				
				
				
				
			

			

				
				Above is a slide of DNA in an agarose gel.  Sudents working with me  run hundreds of these gels.
				
				
				
			

			

				
				
			

			

				Above are two thermocyclers. I helped design the one on the right, which I think out-performs the other. Students working in my lab use of this and all my personally owned equipment.
					

						
				
			

			

				
				
				
				
			

			

				This is my microscope and micromanipulation equipment. I could have bought a sailboat instead because it was bought with my personal money. However, I made the correct decision since I enjoy research a bit more than sailing.
					

						
				
			

			

				
				
				
				
				
				
			

			

				This is taking a biopsy from a microscopic embryo. I was the first in the world to remove just a cell or two for genetic testing.
					

						
				
			

			

				
				
				
				
				
				
				
				
			

			

				When these two halves grow up to be calves, they will be identical twins.
					

						
				
			

			

				
				
			

			

				With this invention, we could collect all the live sperm and leave all the dead ones behind.
					

						
				
			

			

				
				
				
				
				
			

			

				
				Four for the price of one? :: A sheep embryo in the process of being quartered.
					

						
				
			

			

				
				
				
				
				
				
			

			

				
				It must have worked.
					

						
				
			

			

				
				
			

			

				How late in development can embryos be divided and still produce healthy offspring?
					

						
				
			

			

				
				
			

			

				These are progeny generated as a result of splitting embryos 3-5 days after hatching out of their shells.
					

						
				
				
			

			

				
				
				
				
				
			

			

				
				This is a liquid nitrogen tank where embryos and semen can be store indefinitely.
					

						
				
			

			

				
				
				
				
			

			

				In order to determine the sex of cells, first Y-chromosome specific DNA sequences had to be isolated and then sequenced.